随机光学重构显微术及其应用研究进展

杨建宇; 潘雷霆; 胡芬; 张心正; 许京军

doi:10.3788/IRLA201746.1103008

随机光学重构显微术及其应用研究进展

doi: 10.3788/IRLA201746.1103008

1.
南开大学物理科学学院泰达应用物理研究院弱光非线性光子学教育部重点实验室,天津 300071;
2.
南开大学生物治疗协同创新中心,天津 300071

基金项目:

国家自然科学基金（11574165）;111计划（B07013）;长江学者和创新团队发展计划资助课题（IRT_13R29）

详细信息

作者简介:
杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

中图分类号: O439

Stochastic Optical Reconstruction Microscopy and its application

1.
Key Laboratory of Weak-Light Nonlinear Photonics,Ministry of Education,TEDA Institute of Applied Physics,School of Physics,Nankai University,Tianjin 300071,China;
2.
The 2011 Project Collaborative Innovation Center for Biological Therapy,Nankai University,Tianjin 300071,China

摘要: 在光学显微成像领域，涌现出一批可以突破衍射极限的超分辨显微成像技术，极大地增强了人们研究亚细胞结构的能力。基于单分子定位技术的随机光学重构显微术（Stochastic Optical Reconstruction Microscopy，STORM）具有易懂的成像原理、简单的工作方式以及超高的分辨率等特点，受到越来越多的研究者青睐。首先，介绍了单分子定位技术的原理，讨论了STORM光路的搭建，阐述了二维和三维STORM超分辨显微成像原理。其次，探讨了多色STORM以及STORM与电镜关联成像现状。最后介绍了STORM技术现阶段的应用进展。
- 超分辨成像 /
- 单分子定位 /
- 随机光学重构显微术 /
- 关联成像
Abstract: A batch of super-resolution fluorescence microscopy technologies have been invented to overcome the diffraction limit of traditional optical microscopy. It thereby greatly enhances the capacity of sub-cellular structure investigation. Among these super-resolution microscopy, Stochastic Optical Reconstruction Microscopy (STORM) based on single molecule localization has attracted more and more attention by researchers due to its straightforward principle, simple operation mode as well as super-high resolution. First, the basic principle of single molecule localization was introduced, the design of the light path of STORM was discussed, and the principles of 2D-STORM and 3D-STORM were provided. Then, the development of multi-color imaging as well as correlative STORM and electron microscopy were discussed. Finally, some recent typical researches depending on STORM were presented.
- super-resolution imaging /
- single molecule localization /
- Stochastic Optical Reconstruction Microscopy /
- correlative imaging

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随机光学重构显微术及其应用研究进展

doi: 10.3788/IRLA201746.1103008

作者简介:
杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

Stochastic Optical Reconstruction Microscopy and its application

计量

随机光学重构显微术及其应用研究进展

doi: 10.3788/IRLA201746.1103008

1. 南开大学物理科学学院泰达应用物理研究院弱光非线性光子学教育部重点实验室,天津 300071;

2. 南开大学生物治疗协同创新中心,天津 300071

作者简介:
杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

English Abstract

Stochastic Optical Reconstruction Microscopy and its application

1. Key Laboratory of Weak-Light Nonlinear Photonics,Ministry of Education,TEDA Institute of Applied Physics,School of Physics,Nankai University,Tianjin 300071,China;

2. The 2011 Project Collaborative Innovation Center for Biological Therapy,Nankai University,Tianjin 300071,China

全文HTML

目录

留言板

随机光学重构显微术及其应用研究进展

doi: 10.3788/IRLA201746.1103008

作者简介: 杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

Stochastic Optical Reconstruction Microscopy and its application

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出版历程

随机光学重构显微术及其应用研究进展

doi: 10.3788/IRLA201746.1103008

1. 南开大学 物理科学学院 泰达应用物理研究院 弱光非线性光子学教育部重点实验室,天津 300071; 2. 南开大学 生物治疗协同创新中心,天津 300071

作者简介: 杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

English Abstract

Stochastic Optical Reconstruction Microscopy and its application

1. Key Laboratory of Weak-Light Nonlinear Photonics,Ministry of Education,TEDA Institute of Applied Physics,School of Physics,Nankai University,Tianjin 300071,China; 2. The 2011 Project Collaborative Innovation Center for Biological Therapy,Nankai University,Tianjin 300071,China

全文HTML

目录

作者简介:
杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

1. 南开大学物理科学学院泰达应用物理研究院弱光非线性光子学教育部重点实验室,天津 300071;

2. 南开大学生物治疗协同创新中心,天津 300071

作者简介:
杨建宇(1993-),男,硕士生,主要从事超分辨成像方面的研究。Email:yangjy@mail.nankai.edu.cn

1. Key Laboratory of Weak-Light Nonlinear Photonics,Ministry of Education,TEDA Institute of Applied Physics,School of Physics,Nankai University,Tianjin 300071,China;

2. The 2011 Project Collaborative Innovation Center for Biological Therapy,Nankai University,Tianjin 300071,China