肺癌细胞及其聚苯乙烯微球模型的光散射研究

林晓钢; 朱濠; 翁凌冬; 宛楠

doi:10.3788/IRLA201792.1033001

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肺癌细胞及其聚苯乙烯微球模型的光散射研究

林晓钢, 朱濠, 翁凌冬, 宛楠

文章导航 > 红外与激光工程 > 2017 > 46(10): 1033001-1033001(6)

林晓钢, 朱濠, 翁凌冬, 宛楠. 肺癌细胞及其聚苯乙烯微球模型的光散射研究[J]. 红外与激光工程, 2017, 46(10): 1033001-1033001(6). doi: 10.3788/IRLA201792.1033001

引用本文:

林晓钢, 朱濠, 翁凌冬, 宛楠. 肺癌细胞及其聚苯乙烯微球模型的光散射研究[J]. 红外与激光工程, 2017, 46(10): 1033001-1033001(6). doi: 10.3788/IRLA201792.1033001

Lin Xiaogang, Zhu Hao, Weng Lingdong, Wan Nan. Light scattering from lung cancer cells and its Polystyrene microsphere models[J]. Infrared and Laser Engineering, 2017, 46(10): 1033001-1033001(6). doi: 10.3788/IRLA201792.1033001

Citation:

Lin Xiaogang, Zhu Hao, Weng Lingdong, Wan Nan. Light scattering from lung cancer cells and its Polystyrene microsphere models[J]. Infrared and Laser Engineering, 2017, 46(10): 1033001-1033001(6). doi: 10.3788/IRLA201792.1033001

肺癌细胞及其聚苯乙烯微球模型的光散射研究

doi: 10.3788/IRLA201792.1033001

1.
重庆大学光电技术及系统教育部重点实验室,重庆 400044

基金项目:

国家自然科学基金（61377001）

详细信息

作者简介:
林晓钢(1975-),男,副教授,博士,主要从事生物医学光子方面的研究。Email:xglin@cqu.edu.cn

中图分类号: Q24

Light scattering from lung cancer cells and its Polystyrene microsphere models

1.
Key Laboratory of Optoelectronic Technology and Systems of Ministry of Education of China,Chongqing University,Chongqing 400044,China

摘要: 在生物医学和临床医学领域，许多疾病的诊断和治疗依赖于细胞形态的识别。不同细胞具有不同的形态，这些形态的不同将导致生物组织中光传播特征的变化，更重要的是这将影响细胞的光散射特性。目前，动态光散射理论是动态识别细胞尺寸和形状的最佳方式。细胞主要由细胞质、细胞核和线粒体组成，因此，分析它们的光散射特性对于光学诊断和治疗具有非常重要的意义。设计实验获取了癌细胞和聚苯乙烯球的光散射特性，并利用时域有限差分法建立细胞质模型进行细胞光散射特性仿真。从肺癌细胞的光散射结果可以看出，线粒体对前向散射（0~20）和后向散射（160~180）贡献最大，细胞核对侧向散射（80~100）贡献最大，细胞质对各个角度贡献均等。仿真结果和实验结果基本一致。
- 光散射 /
- 数值仿真 /
- 聚苯乙烯微球 /
- 肺癌细胞
Abstract: In the field of biological medicine and clinical medicine, the diagnosis and treatment of many diseases almost rely on the identification of cell morphology. Different cells have different shape which would lead to the change of the light propagation characteristics among biological tissues. What's more, it affects the light scattering properties of cells. At present, the theory of dynamic light scattering is the optimal way to dynamically identify the distribution of the size and the shape which related to the cell. Cells are mainly composed of cytoplasm, nucleus and mitochondria. Therefore, analyzing their optical properties have great significance for optical diagnostic and treatment. Experiments were designed to obtain the light scattering properties of lung cancer cells and Polystyrene microspheres which contained cytoplasm, nucleus and mitochondria. The models of cytoplasm were built with finite different time domain(FDTD) algorithm to simulate the light scattering properties. The light scattering properties of lung cancer cells demonstrate that mitochondria make a contribution to forward scattering (0-20) and backward scattering (160-180), nucleus make a big difference to side scattering (80-100), cytoplasm have an effect on any angle. The result of the simulation testified that the experimental results are correct.
- light scattering /
- numerical simulation /
- Polystyrene microsphere /
- lung cancer cell

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肺癌细胞及其聚苯乙烯微球模型的光散射研究

doi: 10.3788/IRLA201792.1033001

1. 重庆大学光电技术及系统教育部重点实验室,重庆 400044

作者简介:
林晓钢(1975-),男,副教授,博士,主要从事生物医学光子方面的研究。Email:xglin@cqu.edu.cn

收稿日期: 2017-04-10
修回日期: 2017-05-20
刊出日期: 2017-10-25

基金项目:

国家自然科学基金（61377001）

中图分类号: Q24

关键词:

摘要: 在生物医学和临床医学领域，许多疾病的诊断和治疗依赖于细胞形态的识别。不同细胞具有不同的形态，这些形态的不同将导致生物组织中光传播特征的变化，更重要的是这将影响细胞的光散射特性。目前，动态光散射理论是动态识别细胞尺寸和形状的最佳方式。细胞主要由细胞质、细胞核和线粒体组成，因此，分析它们的光散射特性对于光学诊断和治疗具有非常重要的意义。设计实验获取了癌细胞和聚苯乙烯球的光散射特性，并利用时域有限差分法建立细胞质模型进行细胞光散射特性仿真。从肺癌细胞的光散射结果可以看出，线粒体对前向散射（0~20）和后向散射（160~180）贡献最大，细胞核对侧向散射（80~100）贡献最大，细胞质对各个角度贡献均等。仿真结果和实验结果基本一致。

English Abstract

Light scattering from lung cancer cells and its Polystyrene microsphere models

1. Key Laboratory of Optoelectronic Technology and Systems of Ministry of Education of China,Chongqing University,Chongqing 400044,China

Received Date: 2017-04-10
Rev Recd Date: 2017-05-20
Publish Date: 2017-10-25

Keywords:

Abstract: In the field of biological medicine and clinical medicine, the diagnosis and treatment of many diseases almost rely on the identification of cell morphology. Different cells have different shape which would lead to the change of the light propagation characteristics among biological tissues. What's more, it affects the light scattering properties of cells. At present, the theory of dynamic light scattering is the optimal way to dynamically identify the distribution of the size and the shape which related to the cell. Cells are mainly composed of cytoplasm, nucleus and mitochondria. Therefore, analyzing their optical properties have great significance for optical diagnostic and treatment. Experiments were designed to obtain the light scattering properties of lung cancer cells and Polystyrene microspheres which contained cytoplasm, nucleus and mitochondria. The models of cytoplasm were built with finite different time domain(FDTD) algorithm to simulate the light scattering properties. The light scattering properties of lung cancer cells demonstrate that mitochondria make a contribution to forward scattering (0-20) and backward scattering (160-180), nucleus make a big difference to side scattering (80-100), cytoplasm have an effect on any angle. The result of the simulation testified that the experimental results are correct.