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Wang Xin, Wu Jinglin, Fan Xianguang, Xu Yingjie, Lu Xiancong, Zuo Yong. Design of Raman spectroscopy measurement system based on shifted excitation method using two laser diodes with different wavelengths[J]. Infrared and Laser Engineering, 2016, 45(1): 106005-0106005(6). doi: 10.3788/IRLA201645.0106005
Citation: Wang Xin, Wu Jinglin, Fan Xianguang, Xu Yingjie, Lu Xiancong, Zuo Yong. Design of Raman spectroscopy measurement system based on shifted excitation method using two laser diodes with different wavelengths[J]. Infrared and Laser Engineering, 2016, 45(1): 106005-0106005(6). doi: 10.3788/IRLA201645.0106005
shu

Design of Raman spectroscopy measurement system based on shifted excitation method using two laser diodes with different wavelengths

doi: 10.3788/IRLA201645.0106005
  • 1.

    School of Aerospace Engineering,Xiamen University,Xiamen 361005,China;

  • 2.

    The 1st Metrology & Measurement Research Center of National Defense Science Industry of China,Changcheng Institute of Metrology & Measurement,Beijing 100095,China

  • Received Date: 2015-05-08
  • Rev Recd Date: 2015-06-09
  • Publish Date: 2016-01-25
  • Raman detection is often disturbed by fluorescence background, while Shifted Excitation Raman Difference Spectroscopy(SERDS)is an effective method for fluorescent suppression on Raman spectroscopy. Based on this method, a Raman spectroscopy measurement system was designed using two closely space fixed-wavelength laser diodes stabilized with the Volume Bragg Gratings. The output wavelength of laser diodes was stabilized by manipulating their power and temperature. The light path and circuit of spectral data acquisition were designed with back-thinned area array CCD with high sensitivity. Besides, the three different reconstruction algorithms of the difference spectrum, namely simple integration algorithm, simple integration with data interpolation algorithm and multiple energy constraint iterative deconvolution algorithm could be realized through software of this system. The Raman spectra of sesame oil of some brand in the presence of a highly fluorescent were measured with this system and then the spectra reconstructed with three different algorithms to process the difference spectrum respectively were compared. Experimental results show that the system designed in this paper can effectively reject the effect of fluorescence to the Raman spectroscopy measurement.
  • [1] Fan Xianguang, Wang Xin, Xu Yingjie, et al. Design of Raman spectroscopy measurement system based on SHINERS[J]. Infrared and Laser Engineering, 2013, 42(7): 1798-1803. (in Chinese) 范贤光, 王昕, 许英杰, 等。壳层隔绝纳米粒子增强拉曼光谱测试系统设计[J]. 红外与激光工程, 2013, 42(7): 1798-1803.
    [2] Wu B, Chen K, Wang H. Composition dependence of molecular interactions in ethanol-water mixture studied by laser Raman spectra[J]. Infrared and Laser Engineering, 2013, 42(S1): 68-73.(in Chinese)
    [3] Matousek P, Towrie M, Stanley A, et al. Efficient rejection of fluorescence from Raman spectra using picosecond Kerr gating[J]. Applied Spectroscopy, 1999, 53(12): 1485-1489.
    [4] Chase B. FT-Raman spectroscopy: a catalyst for the Raman explosion[J]. Journal of Chemical Education, 2007, 84(1): 75-80.
    [5] Mosier-Boss P A, Lieberman S H, Newbery R. Fluorescence rejection in Raman spectroscopy by shifted-spectra, edge detection, and FFT filtering techniques[J]. Applied Spectroscopy, 1995, 49(5): 630-638.
    [6] Zhao J, Lui H, McLean D I, et al. Automated autofluorescence background subtraction algorithm for biomedical Raman spectroscopy[J]. Applied Spectroscopy, 2007, 61(11): 1225-1232.
    [7] Lieber C A, Mahadevan-Jansen A. Automated method for subtraction of fluorescence from biological Raman spectra[J]. Applied Spectroscopy, 2003, 57(11): 1363-1367.
    [8] Bertinetto C G, Vuorinen T. Automatic baseline recognition for the correction of large sets of spectra using continuous wavelet transform and iterative fitting[J]. Applied Spectroscopy, 2014, 68(2): 155-164.
    [9] Shreve A P, Cherepy N J, Mathies R A. Effective rejection of fluorescence interference in Raman spectroscopy using a shifted excitation difference technique[J]. Applied Spectroscopy, 1992, 46(4): 707-711.
    [10] Matousek P, Towrie M, Parker A W. Simple reconstruction algorithm for shifted excitation Raman difference spectroscopy[J]. Applied Spectroscopy, 2005, 59(6): 848-851.
    [11] Zhao J, Carrabba M M, Allen F S. Automated fluorescence rejection using shifted excitation Raman difference spectroscopy[J]. Applied Spectroscopy, 2002, 56(7): 834-845.
    [12] Osticioli I, Zoppi A, Castellucci E M. Fluorescence and Raman spectra on painting materials: reconstruction of spectra with mathematical methods[J]. Journal of Raman Spectroscopy, 2006, 37(10): 974-980.
    [13] Osticioli I, Zoppi A, Castellucci E M. Shift-excitation raman difference spectroscopy-difference deconvolution method for the luminescence background rejection from raman spectra of solid samples[J]. Applied Spectroscopy, 2007, 61(8): 839-844.
    [14] Martins M A S, Ribeiro D G, Pereira dos Santos E A, et al. Shifted-excitation Raman difference spectroscopy for in vitro and in vivo biological samples analysis[J]. Biomedical Optics Express, 2010, 1(2): 617-626.
    [15] Cai Z, Zou W, Wu J. A dual-excitation difference technique for fluorescence rejection in Raman spectroscopy[C]// Bioelectronics and Bioinformatics(ISBB), 2011 International Symposium on IEEE, 2011: 29-32.
    [16] Kasha M. Characterization of electronic transitions in complex molecules[J]. Discuss Faraday Soc, 1950, 9: 14-19.
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Design of Raman spectroscopy measurement system based on shifted excitation method using two laser diodes with different wavelengths

doi: 10.3788/IRLA201645.0106005
  • 1. School of Aerospace Engineering,Xiamen University,Xiamen 361005,China;
  • 2. The 1st Metrology & Measurement Research Center of National Defense Science Industry of China,Changcheng Institute of Metrology & Measurement,Beijing 100095,China
  • Received Date: 2015-05-08
  • Rev Recd Date: 2015-06-09
  • Publish Date: 2016-01-25

Abstract: Raman detection is often disturbed by fluorescence background, while Shifted Excitation Raman Difference Spectroscopy(SERDS)is an effective method for fluorescent suppression on Raman spectroscopy. Based on this method, a Raman spectroscopy measurement system was designed using two closely space fixed-wavelength laser diodes stabilized with the Volume Bragg Gratings. The output wavelength of laser diodes was stabilized by manipulating their power and temperature. The light path and circuit of spectral data acquisition were designed with back-thinned area array CCD with high sensitivity. Besides, the three different reconstruction algorithms of the difference spectrum, namely simple integration algorithm, simple integration with data interpolation algorithm and multiple energy constraint iterative deconvolution algorithm could be realized through software of this system. The Raman spectra of sesame oil of some brand in the presence of a highly fluorescent were measured with this system and then the spectra reconstructed with three different algorithms to process the difference spectrum respectively were compared. Experimental results show that the system designed in this paper can effectively reject the effect of fluorescence to the Raman spectroscopy measurement.

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