Two-dimensional narrow band multichannel micro-filter array for real-time compact multispectral fluorescent imaging

Yi Dingrong; Kong Linghua; Liu Ting; Wang Zi; Zhao Yanli; Shen Jiahao

doi:10.3788/IRLA201746.0720004

Volume 46 Issue 7

Aug. 2017

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Yi Dingrong, Kong Linghua, Liu Ting, Wang Zi, Zhao Yanli, Shen Jiahao. Two-dimensional narrow band multichannel micro-filter array for real-time compact multispectral fluorescent imaging[J]. Infrared and Laser Engineering, 2017, 46(7): 720004-0720004(7). doi: 10.3788/IRLA201746.0720004

Citation:

Yi Dingrong, Kong Linghua, Liu Ting, Wang Zi, Zhao Yanli, Shen Jiahao. Two-dimensional narrow band multichannel micro-filter array for real-time compact multispectral fluorescent imaging[J]. Infrared and Laser Engineering, 2017, 46(7): 720004-0720004(7). doi: 10.3788/IRLA201746.0720004

Two-dimensional narrow band multichannel micro-filter array for real-time compact multispectral fluorescent imaging

doi: 10.3788/IRLA201746.0720004

1.
College of Mechanical Engineering and Automation,Huaqiao University,Xiamen 361021,China;
2.
School of Mechanical &Automotive Engineering,Fujian University of Technlogy,Fuzhou 350118,China

Received Date: 2016-11-10
Rev Recd Date: 2016-12-20
Publish Date: 2017-07-25

Abstract

At a single snapshot without any mechanical movement, real-time compact multispectral imaging (MSI) is capable of capturing multiple 2-dimensional (XY) images that are corresponding to different characteristic spectrums () of the target. Real-time compact MSI has desirable features including high efficiency, robust to vibration, and a small volume. A two-dimensional narrow band multichannel micro-filter array (2DNBMFA) is an essential part to apply real-time compact MSI to in-vivo optical pathological diagnostic fluorescent imaging, and ultimately result in a real-time compact multispectral fluorescent imaging technology (MSFI). Firstly, the structural pattern was designed and the important technical parameters of the 2DNBMFA were determined, which was the key element to be used in the real-time compact MSFI. Secondly, the 2DNBMFA was manufactured which had a 2-dimensional spatial resolution and a high optical density. Experimental results indicate that the developed micro-filter array has a spatial resolution of 52 m52 m, full-width at half-maximum of 24 nm, optical density of 4, weak cross-talk between different optical channels. All these suggest that the real-time compact MSFI technology is technically feasible, which has the potential to capture the 2-dimensinal distribution of multiple fluorescent probes at any instant time. The real-time compact MSFI, if successfully be developed, would have great significance in medical in-vivo diagnostic imaging and in biological research area where it is hot to use multiple fluorescent probes as biological markers for a better understanding of various life processes.
- 2-dimensional narrow band multichannel filter array,
- micro-filter array,
- real-time compact,
- multi-spectral fluorescent imaging,
- multiple fluorescent probes

References

[1]	Wang Wei, Sun Yu'e, Zhou Naikang, et al. Laser-induced autofluorescence spectroscopy of normal lung, normal bronchus and lung cancer[J]. Chin J Laser Med Surg, 2000, 9(3):137-140. (in Chinese)王伟, 孙玉鹗, 周乃康, 等. 正常肺、支气管组织与肺癌组织激光诱发自体荧光光谱[J]. 中国激光医学杂志, 2000, 9(3):137-140.
[2]	Chen Yajuan, Li Jiaze, Yu Xiaomin. Imaging method of laser induced fluorescence for diagnosing lung cancer[J]. Optical Technique, 2004, 4:452-454. (in Chinese)陈亚娟, 李家泽, 余小敏.激光诱导自体荧光成像法(LIFI)诊断肺癌[J]. 光学技术, 2004, 4:452-454.
[3]	Qiu Zhongfeng, Cui Tingwei, He Yijun. Retrieve of red tide distributions from MODIS data based on the characteristics of water spectrum[J]. Spectroscopy and Spectral Analysis, 2011, 31(8):2233-2237. (in Chinese)丘仲锋, 崔廷伟, 何宜军. 基于水体光谱特性的赤潮分布信息MODIS遥感提取[J].光谱学与光谱分析, 2011, 31(8):2233-2237.
[4]	Ana Herrero-Langreo, Loredana Lunadei, Lourdes Lleo, et al. Multispectral vision for monitoring peach ripeness[J]. Journal of Food Science, 2011, 76(2):E178-E187.
[5]	Xu Hong, Wang Xiangjun. Applications of multispectral/hyperspectral imaging technologies in military[J]. Infrared and Laser Engineering, 2007, 34(1):13-17. (in Chinese)许洪, 王向军. 多光谱、超光谱成像技术在军事上的应用[J]. 红外与激光工程, 2007, 34(1):13-17.
[6]	Wang Xinquan, Xiangli Bin, Huang Min, et al. Static imaging spectropolarimeter[J]. Journal of OptoelectronicsLaser, 2011, 22(5):689-692. 王新全, 相里斌, 黄旻, 等. 静态成像光谱偏振仪[J]. 光电子激光, 2011(5):689-692.
[7]	Zhang Chunmin, Wu Haiying, Li Jie. Fourier transform hyperspectral imaging polarimeter for remote sensing[J]. Optical Engineering, 2011, 50(6):066201.
[8]	Ann M Locke, Derek S Sabatke, Eustace L Dereniak, et al. Snapshot imaging spectropolarimeter[C]//Conference on Polarization Analysis, 2002, 4481:64-72.
[9]	Zhang Yu. Research on snapshot imaging spectral technologies base on micro-lens array[D]. Harbin:Harbin Institute of Technology, 2014:73. (in Chinese)张宇. 基于微透镜阵列的快照式成像光谱技术研究[D]. 哈尔滨:哈尔滨工业大学, 2014:73.
[10]	Kong Linghua, Yi Dingrong, Stephen Sprigle, et al. Single sensor that outputs narrowband multispectral images[J]. Journal of Biomedical Optics, 2010, 15(1):010502.
[11]	Yi Dingrong, Kong Linghua, Wang Fengtao, et al. Instrument an off-shelf CCD imaging sensor into a multispectral imaging bruises detector[J]. IEEE Photonics Technology Letter, 2011, 23(10):606-608.
[12]	Sung K Chang, Yvette N Mirabal, Edward Neely Atkinson, et al. Combined reflectance and fluorescence spectroscopy for in vivo detection of cervical pre-cancer[J]. Journal of Biomedical Optics, 2005, 10(2):024031.
[13]	Mary F Parker, Gregory C Mooradian, Gordon S Okimoto, et al. Initial neural net construction for the detection of cervical intraepithelial neoplasia by fluorescence imaging[J]. American Journal of Obstetrics and Gynecology, 2002, 187(2):398-402.
[14]	Juan Benavides, Sung Chang, Sun Park, et al. Multispectral digital colposcopy for in vivo detection of cervical cancer[J]. Optics Express, 2003, 11(10):1223-1236.
[15]	Yi Dingrong, Kong Linghua. Fabrication of densely patterned micro-arrayed multichannel optical filter mosaic[J]. Journal of Micro/Nanolithography, MEMS, and MOEMS, 2011, 10(3):033020.
[16]	Yi Dingrong, Wang Chao, Qi Hairong, et al. Real-time multispectral imager for home based health care[J]. IEEE Transactions on Biomedical Engineering, 2011, 58(3):736-740.
[17]	Fang Yu, Lv Qunbo, Liu Yangyang, et al. Design and deformation analysis of the filter-array in the filter-array multi-spectral camera[J]. Acta Photonica Sinica, 2015, 44(7):712002-712007. (in Chinese)方煜, 吕群波, 刘扬阳, 等. 滤光片阵列型多光谱相机中阵列的设计与形变影响分析[J]. 光子学报, 2015, 44(7):712002-712007.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Two-dimensional narrow band multichannel micro-filter array for real-time compact multispectral fluorescent imaging

1. College of Mechanical Engineering and Automation,Huaqiao University,Xiamen 361021,China;

2. School of Mechanical &Automotive Engineering,Fujian University of Technlogy,Fuzhou 350118,China

Received Date: 2016-11-10

Rev Recd Date: 2016-12-20

Publish Date: 2017-07-25

Key words:

2-dimensional narrow band multichannel filter array /
micro-filter array /
real-time compact /
multi-spectral fluorescent imaging /
multiple fluorescent probes

Abstract: At a single snapshot without any mechanical movement, real-time compact multispectral imaging (MSI) is capable of capturing multiple 2-dimensional (XY) images that are corresponding to different characteristic spectrums () of the target. Real-time compact MSI has desirable features including high efficiency, robust to vibration, and a small volume. A two-dimensional narrow band multichannel micro-filter array (2DNBMFA) is an essential part to apply real-time compact MSI to in-vivo optical pathological diagnostic fluorescent imaging, and ultimately result in a real-time compact multispectral fluorescent imaging technology (MSFI). Firstly, the structural pattern was designed and the important technical parameters of the 2DNBMFA were determined, which was the key element to be used in the real-time compact MSFI. Secondly, the 2DNBMFA was manufactured which had a 2-dimensional spatial resolution and a high optical density. Experimental results indicate that the developed micro-filter array has a spatial resolution of 52 m52 m, full-width at half-maximum of 24 nm, optical density of 4, weak cross-talk between different optical channels. All these suggest that the real-time compact MSFI technology is technically feasible, which has the potential to capture the 2-dimensinal distribution of multiple fluorescent probes at any instant time. The real-time compact MSFI, if successfully be developed, would have great significance in medical in-vivo diagnostic imaging and in biological research area where it is hot to use multiple fluorescent probes as biological markers for a better understanding of various life processes.

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Reference (17)

[1]	Wang Wei, Sun Yu'e, Zhou Naikang, et al. Laser-induced autofluorescence spectroscopy of normal lung, normal bronchus and lung cancer[J]. Chin J Laser Med Surg, 2000, 9(3):137-140. (in Chinese)王伟, 孙玉鹗, 周乃康, 等. 正常肺、支气管组织与肺癌组织激光诱发自体荧光光谱[J]. 中国激光医学杂志, 2000, 9(3):137-140.
[2]	Chen Yajuan, Li Jiaze, Yu Xiaomin. Imaging method of laser induced fluorescence for diagnosing lung cancer[J]. Optical Technique, 2004, 4:452-454. (in Chinese)陈亚娟, 李家泽, 余小敏.激光诱导自体荧光成像法(LIFI)诊断肺癌[J]. 光学技术, 2004, 4:452-454.
[3]	Qiu Zhongfeng, Cui Tingwei, He Yijun. Retrieve of red tide distributions from MODIS data based on the characteristics of water spectrum[J]. Spectroscopy and Spectral Analysis, 2011, 31(8):2233-2237. (in Chinese)丘仲锋, 崔廷伟, 何宜军. 基于水体光谱特性的赤潮分布信息MODIS遥感提取[J].光谱学与光谱分析, 2011, 31(8):2233-2237.
[4]	Ana Herrero-Langreo, Loredana Lunadei, Lourdes Lleo, et al. Multispectral vision for monitoring peach ripeness[J]. Journal of Food Science, 2011, 76(2):E178-E187.
[5]	Xu Hong, Wang Xiangjun. Applications of multispectral/hyperspectral imaging technologies in military[J]. Infrared and Laser Engineering, 2007, 34(1):13-17. (in Chinese)许洪, 王向军. 多光谱、超光谱成像技术在军事上的应用[J]. 红外与激光工程, 2007, 34(1):13-17.
[6]	Wang Xinquan, Xiangli Bin, Huang Min, et al. Static imaging spectropolarimeter[J]. Journal of OptoelectronicsLaser, 2011, 22(5):689-692. 王新全, 相里斌, 黄旻, 等. 静态成像光谱偏振仪[J]. 光电子激光, 2011(5):689-692.
[7]	Zhang Chunmin, Wu Haiying, Li Jie. Fourier transform hyperspectral imaging polarimeter for remote sensing[J]. Optical Engineering, 2011, 50(6):066201.
[8]	Ann M Locke, Derek S Sabatke, Eustace L Dereniak, et al. Snapshot imaging spectropolarimeter[C]//Conference on Polarization Analysis, 2002, 4481:64-72.
[9]	Zhang Yu. Research on snapshot imaging spectral technologies base on micro-lens array[D]. Harbin:Harbin Institute of Technology, 2014:73. (in Chinese)张宇. 基于微透镜阵列的快照式成像光谱技术研究[D]. 哈尔滨:哈尔滨工业大学, 2014:73.
[10]	Kong Linghua, Yi Dingrong, Stephen Sprigle, et al. Single sensor that outputs narrowband multispectral images[J]. Journal of Biomedical Optics, 2010, 15(1):010502.
[11]	Yi Dingrong, Kong Linghua, Wang Fengtao, et al. Instrument an off-shelf CCD imaging sensor into a multispectral imaging bruises detector[J]. IEEE Photonics Technology Letter, 2011, 23(10):606-608.
[12]	Sung K Chang, Yvette N Mirabal, Edward Neely Atkinson, et al. Combined reflectance and fluorescence spectroscopy for in vivo detection of cervical pre-cancer[J]. Journal of Biomedical Optics, 2005, 10(2):024031.
[13]	Mary F Parker, Gregory C Mooradian, Gordon S Okimoto, et al. Initial neural net construction for the detection of cervical intraepithelial neoplasia by fluorescence imaging[J]. American Journal of Obstetrics and Gynecology, 2002, 187(2):398-402.
[14]	Juan Benavides, Sung Chang, Sun Park, et al. Multispectral digital colposcopy for in vivo detection of cervical cancer[J]. Optics Express, 2003, 11(10):1223-1236.
[15]	Yi Dingrong, Kong Linghua. Fabrication of densely patterned micro-arrayed multichannel optical filter mosaic[J]. Journal of Micro/Nanolithography, MEMS, and MOEMS, 2011, 10(3):033020.
[16]	Yi Dingrong, Wang Chao, Qi Hairong, et al. Real-time multispectral imager for home based health care[J]. IEEE Transactions on Biomedical Engineering, 2011, 58(3):736-740.
[17]	Fang Yu, Lv Qunbo, Liu Yangyang, et al. Design and deformation analysis of the filter-array in the filter-array multi-spectral camera[J]. Acta Photonica Sinica, 2015, 44(7):712002-712007. (in Chinese)方煜, 吕群波, 刘扬阳, 等. 滤光片阵列型多光谱相机中阵列的设计与形变影响分析[J]. 光子学报, 2015, 44(7):712002-712007.

Two-dimensional narrow band multichannel micro-filter array for real-time compact multispectral fluorescent imaging

doi: 10.3788/IRLA201746.0720004

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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