Volume 46 Issue 11
Dec.  2017
Turn off MathJax
Article Contents

Hu Chunguang, Zha Ridong, Ling Qiuyu, He Chengzhi, Li Qifeng, Hu Xiaodong, Hu Xiaotang. Super-resolution microscopy applications and development in living cell[J]. Infrared and Laser Engineering, 2017, 46(11): 1103002-1103002(11). doi: 10.3788/IRLA201746.1103002
Citation: Hu Chunguang, Zha Ridong, Ling Qiuyu, He Chengzhi, Li Qifeng, Hu Xiaodong, Hu Xiaotang. Super-resolution microscopy applications and development in living cell[J]. Infrared and Laser Engineering, 2017, 46(11): 1103002-1103002(11). doi: 10.3788/IRLA201746.1103002

Super-resolution microscopy applications and development in living cell

doi: 10.3788/IRLA201746.1103002
  • Received Date: 2017-10-10
  • Rev Recd Date: 2017-11-20
  • Publish Date: 2017-11-25
  • Cell is the basic unit and functional unit of living body. The study of the internal structure and function of living cells is one of the foundations of mastering the essence of life. Therefore, the realtime observation of living cells is of great significance for the development of life sciences. Conventional optical microscopy is limited by the diffraction limits and can not observe the details of biological structures below 200 nm. In the past 20 years, with the rapid development of super-diffraction limit optical theory, technology, devices and fluorescent probes, super-resolution microscopy has become an important method for life science research. However, most super-resolution microscopic methods or measurements take a long time, or are likely to cause photobleaching/phototoxicity, and are severely limited in living cell studies. In this paper, based on the study of fast super-resolution microscopy, the photoactivated localization microscopy and stochastic optical reconstruction microscopy were introduced based on single molecule localization microscopy. The stimulated emission depletion microscopy based on fluorescence non-linear saturated light conversion and structured illumination microscopy based on structured light illumination was also introduced. Besides, the development and application of cell imaging were explored. Finally, an outlook of the future development trend of super-resolution microscopy in living cell imaging was provided.
  • [1] Stephens D J, Allan V J. Light microscopy techniques for live cell imaging[J]. Science, 2003, 300(5616):82-86.
    [2] Minsky M. Microscopy apparatus:US, 3013467[P]. 1961-12-19.
    [3] Ash E A, Nicholls G. Super-resolution aperture scanning microscopy[J]. Nature, 1972, 237(5357):510-512.
    [4] Binning G, Rohrer H, Gerber C, et al. Surface studies by scanning tunneling microscopy[J]. Physical Review Letters, 1982, 49(1):57-61.
    [5] Axelrod D. Cell-substrate contacts illuminated by total internal reflection fluorescence[J]. Journal of Cell Biology, 1981, 89(1):141-145.
    [6] Yildiz A, Forkey J N, Mckinney S A, et al. Myosin V walks hand-over-hand:single fluorophore imaging with 1.5-nm localization[J]. Science, 2003, 300(5628):2061-2065.
    [7] Betzig E. Proposed method for molecular optical imaging[J].Optics Letters, 1995, 20(3):237-239.
    [8] Betzig E, Patterson G H, Sougrat R, et al. Imaging intracellular fluorescent proteins at nanometer resolution[J].Science, 2006, 313(5793):1642-1645.
    [9] Rust M, Bates M, Zhuang X W. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy(STORM)[J]. Nature Methods, 2006, 3(10):793-795.
    [10] Shroff H, Galbraith C G, Galbraith J A, et al. Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes[J]. Proceedings of the National Academy of Sciences of United States of America, 2007, 104(51):20308-20313.
    [11] Shroff H, Galbraith C G, Galbraith J A, et al. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics[J]. Nature Methods, 2008, 5(5):417-423.
    [12] Bates M, Huang B, Dempsey G T. et al. Multicolor super-resolution imaging with photo-switchable fluorescent probes[J]. Science, 2007, 317(5845):1749-1752.
    [13] Matsuda A, Shao L, Boulanger J, et al. Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP-histones[J]. Plos one, 2010, 5(9):e12768.
    [14] Shtengel G, Galbraith J A, Galbraith C G, et al. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(9):3125-3130.
    [15] Wang Y, Kanchawong P. Three-dimensional super resolution microscopy of factin filaments by interferometric photoactivated localization microscopy[J]. Journal of Visualized Experiments, 2016, 118:e54774.
    [16] Kanchanawong P, Shtengel G, Pasapera A M, et al. Nanoscale architecture of integrain-based cell adhesions[J].Nature, 2010, 468(7323):580-584.
    [17] Jones S A, Shim S H, He J, et al. Fast, three-dimensional super-resolution imaging of live cells[J]. Nature Methods,2011, 8(6):499-505.
    [18] Holden S J, Uphoff S, Kapanidis A N. DAOSTORM:an algorithm for high-density super-resolution microscopy[J].Nature Methods, 2011, 8(4):279-280.
    [19] Babcock H, Sigal Y M, Zhuang X W. A high-density 3D localization algorithm for stochastic optical reconstruction microscopy[J]. Optical Nanoscopy, 2012, 1(1):1-6.
    [20] Huang F, Schwartz S L, Byars J M, et al. Simultaneous multiple-emitteer fitting for single molecule super-resolution imaging[J]. Optic Express, 2011, 2(5):1377-1394.
    [21] Quan T W, Zhu H Y, Long F, et al. High-density localization of fluorescent molecules using Structured Sparse Model and Bayesian Information Criterion[J]. Optic Express, 2011, 19(18):16974.
    [22] Cox S, Rosten E, Monypenny J, et al. Bayesian localization microscopy reveals nanoscale podosome dynamics[J]. Nature Methods, 2012, 9(2):195-200.
    [23] Xu Fan, Zhang Mingshu, He Wenting, et al. Live cell single molecule-guided Bayesian localization super resolution microscopy[J]. Cell Research, 2016, 27(5):713-716.
    [24] Willig K I, Rizzoli S O, Westphal V, et al. STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis[J]. Nature, 2006, 440(7086):935-939.
    [25] Hell S W, Wichmann J. Breaking the diffraction resolution limit by stimulated emission:Stimulated-emission-depletion fluorescence microscopy[J]. Optics Letters, 1994, 19(11):780-782.
    [26] Vicidomini G, Moneron G, Han K Y, et al. Sharper low-power STED nanoscopy by time gating[J]. Nature Methods, 2011, 8(7):571-575.
    [27] Hao Xiang, Kuang Cuifang, Gu Zhaotai, et al. Super resolution microscuopy of offline g-STED nanoscopy based on time-correlated single photon counting[J]. Chin J Lasers, 2013, 40(1):0104001. 郝翔, 匡翠方, 顾兆泰, 等.基于时间相关单光子计数的离线式g-STED超分辨显微技术[J]. 中国激光, 2013, 40(1):0104001.
    [28] Hernandez I C, Castello M, Lanzano L, et al. Two-photon excitation STED microscopy with time-gated detection[J].Scientific Reports, 2016, 6:19419.
    [29] Vicidomini G, Schonie A, Han K Y, et al. STED nanoscopy with time-gated detection:theoretical and experimental aspects[J]. Plos One, 2013, 8(1):e54421.
    [30] Vicidomini G, Hernandez I C, Damora M, et al. Gated CW-STED microscopy:A versatile tool for biological nanometer scale investigation[J]. Methods, 2014, 66(2):124-130.
    [31] Hao Xiang, Kuang Cuifang, Li Yanghui, et al. Manipulation of doughnut focal spot by image imverting interferometry[J]. Optics Letters, 2012, 37(5):821-823.
    [32] Stender A S, Marchuk K, Liu C, et al. Single cell optical imaging and spectroscopy[J]. Chemical Reviews, 2013, 113(4):2469.
    [33] Westphal V, Rizzoli S O, Lauterbach M A, et al. Video-rate far-field optical nanoscopy dissects synaptic vesicle movement[J]. Science, 2008, 320(5873):246-249.
    [34] Bingen P, Reuss M, Engelhardt J, et al. Parallelized STED fluorescence nanoscopy[J]. Optics Express, 2011, 19(24):23716-23726.
    [35] Yang B, Przybilla F, Mestre M. et al. Large parallelization of STED nanoscopy using optical lattices[J]. Optics Express,2014, 22(5):5581-5589.
    [36] Yang B, Fang C Y, Treussart F, et al. Polarization effects in lattice-STED microscopy[J]. Faraday Discussions, 2015, 184:37-49.
    [37] Helmchen F, Denk W. Deep tissue two-photon microscopy[J]. Nature Methods, 2005, 2(12):932-940.
    [38] Moneron G, Hell S W. Two-photon excitation STED microscopy[J]. Optics Express, 2009, 17(17):14567.
    [39] Scheul T, D'Amico C, Wang I, et al. Two-photon excitation and stimulated emission depletion by a single wavelength[J]. Optics Express, 2011, 19(19):18036-18048.
    [40] Friedrich M, Gan Q, Ermolayev W, et al. STED-SPIM:Stimulated emission depletion improves sheet illumination microscopy resolution[J]. Biophysical Journal, 2011, 100(8):L43-L45.
    [41] Friedrich M, Harms G S. Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion[J]. Journal of Biomedical Optics, 2015, 20(10):106006.
    [42] Heintzmann R, Cremer C. Laterally modulated excitation microscopy:Improvement of resolution by using a diffraction grating[C]//SPIE, 1999, 3568:185-196.
    [43] Gustafsson M G L, Agard D A, Sedat J W. Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination[C]//SPIE, 2000, 3919:141-150.
    [44] Gustafsson M G L, Shao L, Carlton P M, et al. Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination[J]. Biophysical Journal, 2008, 94(12):4957-4970.
    [45] Shao L, Isaac B, Uzawa S, et al. I5S:wide field light microscopy with 100-nm-scale resolution in three dimensions[J]. Biophysical Journal, 2008, 94(12):4971-4983.
    [46] Gustafsson M G L. Nonlinear structured-illumination microscopy:Wide-field fluorescence imaging with theoretically unlimited resolution[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(37):13081-13086.
    [47] Ando R, Mizuno H, Miyawaki A. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting[J]. Science, 2004, 306(5700):1370-1373.
    [48] Habuchi S, Ando R, Dedecker P, et al. Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(37):9511-9516.
    [49] Habuchi S, Dedecker P, Hotta J, et al. Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa:mechanism responsible for the reversible photoswitching[J]. Photochem Photobiological Science, 2006, 5(6):567-576.
    [50] Rego E H, Shao L, Macklin J J, et al. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(3):E135-E143.
    [51] Kner P, Chhun B B, Griffis E R, et al. Super-resolution video microscopy of live cells by structured illumination[J]. Nature Methods, 2009, 6(5):339-342.
    [52] Shao L, Kner P, Rego E H, et al. Super-resolution 3D microscopy of live whole cells using structured illumination[J]. Nature Methods, 2011, 8(12):1044-1046.
    [53] York A G, Parekh S H, Dalle Nogare D, et al. Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy[J]. Nature Methods, 2012, 9(7):749-754.
    [54] Schulz O, Pieper C, Clever M, et al. Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanningmicroscopy[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(52):21000-21005.
    [55] York A G, Chandris P, Nogare D D, et al. Instant super-resolution imaging in live cells and embryos via analog image processing[J]. Nature Methods, 2013, 10(11):1122-1126.
    [56] Gao L, Shao L, Chen B C, et al. 3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy[J]. Nature Protocls, 2014, 9(5):1083-1101.
    [57] Chang B J, Meza V D P, Stelzer E H K. csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm[J]. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(19):4869.
    [58] Chen B C, Legant W R, Wang K, et al. Lattice light-sheet microscopy:imaging molecules to embryos at high spatio-temporal resolution[J]. Science, 2014, 346(6208):1257998.
    [59] Dibg Li, Lin Shao, Chen Bichang, et al. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics[J]. Science, 2015, 349(6251):6251.
    [60] Legant W R, Shao L, Grimm J B, et al. High-density three-dimensional localization microscopy across large volumes[J]. Nature Methods, 2016, 13(4):359-365.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(635) PDF downloads(428) Cited by()

Related
Proportional views

Super-resolution microscopy applications and development in living cell

doi: 10.3788/IRLA201746.1103002
  • 1. School of Precision Instrument &Opto-Electronics Engineering,Tianjin University,Tianjin 300072,China

Abstract: Cell is the basic unit and functional unit of living body. The study of the internal structure and function of living cells is one of the foundations of mastering the essence of life. Therefore, the realtime observation of living cells is of great significance for the development of life sciences. Conventional optical microscopy is limited by the diffraction limits and can not observe the details of biological structures below 200 nm. In the past 20 years, with the rapid development of super-diffraction limit optical theory, technology, devices and fluorescent probes, super-resolution microscopy has become an important method for life science research. However, most super-resolution microscopic methods or measurements take a long time, or are likely to cause photobleaching/phototoxicity, and are severely limited in living cell studies. In this paper, based on the study of fast super-resolution microscopy, the photoactivated localization microscopy and stochastic optical reconstruction microscopy were introduced based on single molecule localization microscopy. The stimulated emission depletion microscopy based on fluorescence non-linear saturated light conversion and structured illumination microscopy based on structured light illumination was also introduced. Besides, the development and application of cell imaging were explored. Finally, an outlook of the future development trend of super-resolution microscopy in living cell imaging was provided.

Reference (60)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return